| Autor: |
Elizaveta A. Rukosueva, Valeria A. Belikova, Ivan N. Krylov, Vladislav S. Orekhov, Evgenii V. Skorobogatov, Andrei V. Garmash, Mikhail K. Beklemishev |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Sensors, Vol 20, Iss 18, p 5351 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1424-8220 |
| DOI: |
10.3390/s20185351 |
| Popis: |
In this study we develop a variant of fluorescent sensor array technique based on addition of fluorophores to samples. A correct choice of fluorophores is critical for the successful application of the technique, which calls for the necessity of comparing different discrimination protocols. We used 36 honey samples from different sources to which various fluorophores were added (tris-(2,2′-bipyridyl) dichlororuthenium(II) (Ru(bpy)32+), zinc(II) 8-hydroxyquinoline-5-sulfonate (8-Ox-Zn), and thiazole orange in the presence of two types of deoxyribonucleic acid). The fluorescence spectra were obtained within 400–600 nm and treated by principal component analysis (PCA). No fluorophore allowed for the discrimination of all samples. To evaluate the discrimination performance of fluorophores, we introduced crossing number (CrN) calculated as the number of mutual intersections of confidence ellipses in the PCA scores plots, and relative position (RP) characterized by the pairwise mutual location of group centers and their most distant points. CrN and RP parameters correlated with each other, with total sensitivity (TS) calculated by Mahalanobis distances, and with the overall rating based on all metrics, with coefficients of correlation over 0.7. Most of the considered parameters gave the first place in the discrimination performance to Ru(bpy)32+ fluorophore. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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