Genetic relationships and diversity among wild Salvia accessions revealed by ISSR and SCoT markers

Autor: Alireza Etminan, Alireza Pour-Aboughadareh, Afsaneh Noori, Amin Ahmadi-Rad, Lia Shooshtari, Zahra Mahdavian, Masoumeh Yousefiazar-Khanian
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Biotechnology & Biotechnological Equipment, Vol 32, Iss 3, Pp 610-617 (2018)
Druh dokumentu: article
ISSN: 1310-2818
1314-3530
13102818
DOI: 10.1080/13102818.2018.1447397
Popis: In this study, inter-simple sequence repeats (ISSR) and start codon targeted (SCoT) markers were used for genetic diversity and relationship analysis of nine Salvia species. Twenty-one and twenty selected ISSR and SCoT primers amplified 350 and 329 loci, respectively, of which all were polymorphic. The obtained average polymorphism information content (ISSR, 0.38; SCoT, 0.40), average band informativeness (ISSR, 16.67; SCoT, 16.45) and resolving power (ISSR, 9.75; SCoT, 12.52) revealed high genetic diversity prevailing among Salvia accessions. Considering the ISSR and SCoT data, the species with a basic chromosome number of x = 8 showed higher values of the percentage polymorphism loci (PPL), the number of observed alleles (Na) and Shannon index (I) than the other species. The partition of clusters in the neighbour-joining dendrogram based on ISSR, SCoT and combined data was similar and grouped all individuals into four clusters. However, the dendrogram generated based on SCoT separated the individuals into sub-clusters in accordance with their species and section. The Mantel test revealed a similar polymorphism distribution pattern between ISSR and SCoT techniques, the correlation coefficient (r) was 0.83, and the results showed that both techniques were effective to assess the genetic diversity. Our results indicated that SCoT markers can be used as a reliable and informative technique for evaluation of genetic diversity and relationships among Salvia species.
Databáze: Directory of Open Access Journals
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