The Effect of Intravitreal Bevacizumab on Apoptosis of Rat Retina Cells
| Autor: | Özcan Kayıkçıoğlu, Seda Vatansever, İsmet Topçu, Işıl Aydemir, Tuğçe Bilecenoğlu, Fatih Kahvecioğlu, Sinan Emre |
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| Jazyk: | English<br />Turkish |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Türk Oftalmoloji Dergisi, Vol 43, Iss 1, Pp 39-44 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1300-0659 2147-2661 |
| DOI: | 10.4274/tjo.43.07279 |
| Popis: | Pur po se: To investigate the apoptotic effects of intravitreal bevacizumab on rat retinal cells. Ma te ri al and Met hod: Thirty-six male adult Swiss albino rats were randomly divided into two groups. The first group was applied 0.25 mg bevacizumab in 0.01 ml saline, and the second group received the same amount of saline intravitreally. Each group was divided into 3 subgroups. The animals were sacrificed and their globes were enucleated at the 3rd, 24th, and 72nd hours of the experiment. Enucleated eyes were preserved for histological analysis, immunohistochemical analysis of caspase-3, caspase-8, caspase-9, Fas/Fas L, VEGF and VEGF receptors (Flt-1, Flk-1), and TUNEL staining. Re sults: Histological evaluation showed no sign of retinal toxicity in both groups. In TUNEL staining, TUNEL(+) cells were detected in all subgroups, but the number of positive cells was relatively lower in bevacizumab treatment group that reached statistically significant level at 24 and 72 hours of treatment (p |
| Databáze: | Directory of Open Access Journals |
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