| Autor: |
Gun-Soo Park, Seung-Hwa Baek, Keunbon Ku, Seong Jun Kim, Seung Il Kim, Bum-Tae Kim, Jin-Soo Maeng |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Bio-Protocol, Vol 10, Iss 21 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2331-8325 |
| DOI: |
10.21769/BioProtoc.3804 |
| Popis: |
Standard diagnostic methods of Coronavirus Disease 2019 (COVID-19) rely on RT-qPCR technique which have limited point-of-care test (POCT) potential due to necessity of dedicated equipment and specialized personnel. LAMP, an isothermal nucleic acid amplification test (NAAT), is a promising technique that may substitute RT-qPCR for POCT of genomic materials. Here, we provide a protocol to perform reverse transcription LAMP targeting SARS-CoV-2. We adopted both real-time fluorescence detection and end-point colorimetric detection approaches. Our protocol would be useful for screening diagnosis of COVID-19 and be a baseline for development of improved POCT NAAT. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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