| Autor: |
Qiaojuan Gong, Haixia Han, Haiying Yang, Meiling Zhang, Xiaoling Sun, Yunxia Liang, Zhaorong Liu, Wenchan Zhang, Jinli Qiao |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Journal of Materiomics, Vol 5, Iss 2, Pp 313-319 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2352-8478 |
| DOI: |
10.1016/j.jmat.2019.03.004 |
| Popis: |
A nanocomposite of polyaniline/graphene (PAN/GN) was prepared using reverse-phase polymerization. The nanocomposite material was dropcast onto a glassy carbon electrode (GCE). Then, a single-stranded DNA (ssDNA) probe for HIV-1 gene detection was immobilized on the modified electrode, and the negative charged phosphate backbone of the HIV-1 was bound to the modified electrode surface via π-π∗ stacking interactions. The hybridization between the ssDNA probe and the target HIV-1 formed double-stranded DNA (dsDNA), and the electron transfer resistance of the electrode was measured using impedimetric studies with a [Fe(CN)6]3-/4- redox couple. Under the optimized experimental conditions, the change of the impedance value was linearly related to the logarithm of the concentration of HIV genes in the range from 5.0 × 10−16 M to 1.0 × 10−10 M (R = 0.9930), and the HIV sensor exhibited a lower detection limit of 1.0 × 10−16 M (S/N = 3). The results show that this biosensor presented wonderful selectivity, sensitivity and specificity for HIV-1 gene detection. Thus, this biosensor provides a new method for the detection of HIV gene fragments. Keywords: Graphene, Polyaniline, HIV gene, Electrochemical impedance biosensor |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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