| Autor: |
Md Habibul Hasan Mazumder, Jasleen Gandhi, Nairrita Majumder, Lei Wang, Robert Ian Cumming, Sydney Stradtman, Murugesan Velayutham, Quincy A. Hathaway, Jonathan Shannahan, Gangqing Hu, Timothy R. Nurkiewicz, Robert M. Tighe, Eric E. Kelley, Salik Hussain |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Particle and Fibre Toxicology, Vol 20, Iss 1, Pp 1-21 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1743-8977 |
| DOI: |
10.1186/s12989-023-00528-8 |
| Popis: |
Abstract Background Microbial dysbiosis is a potential mediator of air pollution-induced adverse outcomes. However, a systemic comparison of the lung and gut microbiome alterations and lung-gut axis following air pollution exposure is scant. In this study, we exposed male C57BL/6J mice to inhaled air, CB (10 mg/m3), O3 (2 ppm) or CB + O3 mixture for 3 h/day for either one day or four consecutive days and were euthanized 24 h post last exposure. The lung and gut microbiome were quantified by 16 s sequencing. Results Multiple CB + O3 exposures induced an increase in the lung inflammatory cells (neutrophils, eosinophils and B lymphocytes), reduced absolute bacterial load in the lungs and increased load in the gut. CB + O3 exposure was more potent as it decreased lung microbiome alpha diversity just after a single exposure. CB + O3 co-exposure uniquely increased Clostridiaceae and Prevotellaceae in the lungs. Serum short chain fatty acids (SCFA) (acetate and propionate) were increased significantly only after CB + O3 co-exposure. A significant increase in SCFA producing bacterial families (Ruminococcaceae, Lachnospiraceae, and Eubacterium) were also observed in the gut after multiple exposures. Co-exposure induced significant alterations in the gut derived metabolite receptors/mediator (Gcg, Glp-1r, Cck) mRNA expression. Oxidative stress related mRNA expression in lungs, and oxidant levels in the BALF, serum and gut significantly increased after CB + O3 exposures. Conclusion Our study confirms distinct gut and lung microbiome alterations after CB + O3 inhalation co-exposure and indicate a potential homeostatic shift in the gut microbiome to counter deleterious impacts of environmental exposures on metabolic system. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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