Isolation, characterization and molecular identification of a halotolerant Bacillus megaterium CTBmeg1 able to grow on halogenated compounds

Autor: Kazim Akcay, Yilmaz Kaya
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Biotechnology & Biotechnological Equipment, Vol 33, Iss 1, Pp 945-953 (2019)
Druh dokumentu: article
ISSN: 1310-2818
1314-3530
13102818
DOI: 10.1080/13102818.2019.1631717
Popis: Halogenated compounds pose long-term potential risks to the well-being of humans due to their recalcitrance and persistent toxicity. The quest for microorganisms capable of degrading such perilous substances merits urgent consideration. In this study, a new dehalogenase-producing bacterium was isolated from a hypersaline environment (TuzGölü Lake, Turkey), and identified as Bacillus megaterium strain CTBmeg1 (Accession number MK128900). Under culture conditions (pH 8.0, NaCl 20%, 30 °C, 200 rpm, 9 days), the B. megaterium strain CTBmeg1 showed an optimum growth on 10 mmol/L of 2,2-dichloropropionic acid with a doubling time of 26.41 h. Furthermore, the presence of a putative halotolerant dehalogenase gene (dehCTBmeg1) of B. megaterium strain CTBmeg1 was detected and amplified via PCR technique. Bio-prospecting for microorganisms in a highly saline environment capable of utilizing halogenated compounds as the sole carbon may prove to be a practical and safer means for bioremediation of contaminated coastal areas, an increasingly common place predicament faced by many nations.
Databáze: Directory of Open Access Journals
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