| Autor: |
Xiumei Lin, Xueqian Yang, Chuan Chen, Wen Ma, Yiqi Wang, Xuerong Li, Kaichen Zhao, Qiuting Deng, Weimin Feng, Yuting Ma, Hui Wang, Lveming Zhu, Sunil Kumar Sahu, Fengzhen Chen, Xiuqing Zhang, Zhiqiang Dong, Chuanyu Liu, Longqi Liu, Chang Liu |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Scientific Data, Vol 10, Iss 1, Pp 1-10 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2052-4463 |
| DOI: |
10.1038/s41597-023-02373-y |
| Popis: |
Abstract Vertebrate embryogenesis is a remarkable process, during which numerous cell types of different lineages arise within a short time frame. An overwhelming challenge to understand this process is the lack of dynamic chromatin accessibility information to correlate cis-regulatory elements (CREs) and gene expression within the hierarchy of cell fate decisions. Here, we employed single-nucleus ATAC-seq to generate a chromatin accessibility dataset on the first day of zebrafish embryogenesis, including 3.3 hpf, 5.25 hpf, 6 hpf, 10 hpf, 12 hpf, 18 hpf and 24 hpf, obtained 51,620 high-quality nuclei and 23 clusters. Furthermore, by integrating snATAC-seq data with single-cell RNA-seq data, we described the dynamics of chromatin accessibility and gene expression across developmental time points, which validates the accuracy of the chromatin landscape data. Together, our data could serve as a fundamental resource for revealing the epigenetic regulatory mechanisms of zebrafish embryogenesis. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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