Metabolic response induced by endophytic fungi and bacteria in H. marrubioides Epling in vitro microplants

Autor: Luciana Cristina Vitorino, Fabiano Guimarães Silva, William Cardoso Lima, Marcos Antônio Soares, Rita Cássia Nascimento Pedroso, Marolí Rodrigues Silva, Herbert Júnior Dias, Antônio Eduardo Miller Crotti, Márcio Luís Andrade e Silva, Wilson Roberto Cunha, Patrícia Mendonça Pauletti, Ana Helena Januário
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2013
Předmět:
Zdroj: Química Nova, Vol 36, Iss 7, Pp 1014-1020 (2013)
Druh dokumentu: article
ISSN: 1678-7064
0100-4042
DOI: 10.1590/S0100-40422013000700016
Popis: Hyptis marrubioides Epling is a native plant from Brazilian Cerrado. In this paper, the response of in vitro microplants of this species to inoculation with bacterial and fungal endophytic isolates is evaluated. HPLC-DAD analysis showed the presence of 3,4-O-(Z)-dicaffeoylquinic acid and quercetin-7-O-glucoside as the main components. GC/MS analysis demonstrated that the sesquiterpenes τ-cadinol and caryophyllene oxide were only produced in microplants inoculated with endophytic bacteria, while methyl hexadecanoate, methyl heptadecanoate and methyl (Z,Z,Z) 9,12,15-octadecatrienoate and the triterpene methyl 3β-hydroxy-urs-12-en-28-oate were overexpressed only when the microplant was treated with endophytic fungi.
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