| Autor: |
Jakeen K. El-Jakee, Ihab M. Moussa, Mai S. Omran, Basem M. Ahmed, Mahmoud A. Elgamal, Hassan A. Hemeg, Ayman S. Mubarak, Khalid S. Al-Maary, Saleh A. Kabli, Sherif A. Marouf, Jwaher Haji Alhaaji |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Saudi Journal of Biological Sciences, Vol 27, Iss 3, Pp 996-1001 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1319-562X |
| DOI: |
10.1016/j.sjbs.2019.12.042 |
| Popis: |
In the present study, a bivalent vaccine against Pasteurella multocida and rabbit hemorrhagic disease virus (RHDV) was formulated with Montanide™ ISA70 oil adjuvant (Seppic, Paris, France). Its efficacy was evaluated and compared to similar monovalent preparations and commercially available monovalent vaccines. White new Zeeland rabbit groups (n = 10) received 2 successive doses of the tested vaccines and were challenged 2 weeks after 2nd dose with Pasteurella multocida and RHDV or either pathogens according to their vaccination schedule. Challenged not-vaccinated group of rabbits (n = 10) was included as a control. The bivalent and monovalent ISA70 preparations were found stable, safe, sterile, pure and of low viscosity. Group 3 (GP3) which received bivalent vaccine showed the highest antibody geometric mean titers against Pasteurella multocida and RHDV evaluated by ELISA and hemagglutination inhibition (HI) respectively. Following virulent challenge; Gp3 rabbits were 90% protected from challenge over other groups that showed 80% protection. Detection of either pathogen in the livers of dead and euthanized rabbits had failed except for non-vaccinated controls. The bivalent vaccine candidate was fully protective. Immunization against both pathogens can be achieved by single vaccination. Keywords: Pasteurellosis, RHDV, Vaccine, Rabbits, Bivalent, Montanide ISA70 |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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