| Autor: |
HUANG Gui-zhen, WANG Qing-xiang, CHEN Jin-mei, ZHAN Feng-ping, WEI Lan, ZHENG Wan-rong |
| Jazyk: |
English<br />Chinese |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Shipin yu jixie, Vol 39, Iss 1, Pp 24-30 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1003-5788 |
| DOI: |
10.13652/j.spjx.1003.5788.2022.80552 |
| Popis: |
Objective: Construction of a simple and rapid method for the analysis of ganoderic acid A （GAA）. Methods: The molecular imprinted polymer （MIP） that can specifically recognize ganoderic acid A （GAA） were prepared by cyclic voltammetric deposition using GAA as template molecule and o-phenylenediamine as functional monomer. The structure, morphology and electrochemical behavior of MIP were characterized by infrared spectroscopy, scanning electron microscopy and electrochemical methods. Using [Fe（CN）6]3-/4- as the active indicator, the analytical performance of the sensor for GAA was investigated. Porous MIP for GAA can be prepared on the electrode surface by one-step electropolymerization. Results: The results of quantitative analysis showed that the electrochemical signal of [Fe（CN）6]3-/4- had a good linear relationship with the logarithm of GAA concentration in the range of 1.0 pmol/L～1.0 μmol/L, and the detection limit was 0.21 pmol/L. The measured values of GAA in ethanol and water-soluble solution of Ganoderma lucidum powder were 1.15 nmol/L and 3.00 pmol/L, respectively. Conclusion: The MIP-based GAA electrochemical sensor can be used for the rapid determination of GAA in extract solution of Ganoderma lucidum powder. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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