Biological Control of Sclerotinia Rot Caused by Sclerotinia sclerotiorum in Lettuce Using Bacillus amyloliquefaciens HY

Autor: Mi-Ra Son, Jin-Won Kim
Jazyk: English<br />Korean
Rok vydání: 2024
Předmět:
Zdroj: Research in Plant Disease, Vol 30, Iss 3, Pp 247-255 (2024)
Druh dokumentu: article
ISSN: 1598-2262
2233-9191
DOI: 10.5423/RPD.2024.30.3.247
Popis: To test the antagonistic ability of Bacillus amyloliquefaciens HY against lettuce sclerotinia rot caused by Sclerotinia sclerotiorum, an in vitro test, greenhouse test and growth chamber test were conducted. As a result of in vitro dual culture test for sclerotia of B. amyloliquefaciens HY, the mycelial growth inhibition rate was 70.7%. Mycelial tip was swollen, black, and had an irregular and deformed shape. In I-plate test, the antagonistic volatile compounds produced by B. amyloliquefaciens HY, the mycelial growth inhibition rate was 57.7% and the sclerotia germination inhibition rate was 46.7%. In addition, B. amyloliquefaciens HY had sideropore production capacity, phosphate solubillibty, and protease production capability, but B. amyloliquefaciens HY did not produce chitinase. In growth chamber test, the lettuce survival rate was 0% in the control group treated with only the pathogen, and 80% in the treatment group treated with of S. sclerotiorum and B. amyloliquefaciens HY by diluting 10 times of the lettuce survival rate. In addition, the lettuce survival rate of 80% was shown in the treatment group treated with the culture medium of B. amyloliquefaciens HY 50 times 1 week later after inoculation with S. sclerotiorum.
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