| Autor: |
Pastor Johanne, Zou Yonglong, Jiwani Ameena J, Dong Ying, Wang Xiaoyu, Kuro-o Makoto, Habib Amyn A, Ruan Minzi, Boothman David A, Yang Chin-Rang |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Proteome Science, Vol 9, Iss 1, p 53 (2011) |
| Druh dokumentu: |
article |
| ISSN: |
1477-5956 |
| DOI: |
10.1186/1477-5956-9-53 |
| Popis: |
Abstract An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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