Autor: |
Marielle Mentek, Benjamin Peyret, Siwar Zouari, Sébastien Urbaniak, Jean-Marie Papillon, Emmanuel Crouzet, Chantal Perrache, Sophie Hodin, Xavier Delavenne, Zhiguo He, Philippe Gain, Gilles Thuret |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
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Zdroj: |
International Journal of Pharmaceutics: X, Vol 7, Iss , Pp 100234- (2024) |
Druh dokumentu: |
article |
ISSN: |
2590-1567 |
DOI: |
10.1016/j.ijpx.2024.100234 |
Popis: |
Corneal epithelial barrier represents one of the major limitations to ocular drug delivery and can be explored non-invasively through the evaluation of its electrical properties. Human corneas stored in active storage machine (ASM) could represent an interesting physiological model to explore transcorneal drug penetration. We designed a new system adapted to human corneas preserved in ASM to explore corneal epithelial barrier function ex-vivo. A bipolar set-up including Ag/AgCl electrodes adaptors to fit the corneal ASM and a dedicated software was designed and tested on freshly excised porcine corneas (n = 59) and human corneas stored 14 days in ASM (n = 6). Porcine corneas presented significant and proportional decrease in corneal impedance in response to increasing-size epithelial ulcerations and acute exposure to benzalkonium chloride (BAC) 0.01 and 0.05%. Human corneas stored 14 days in ASM presented a significant increase in corneal impedance associated with the restoration of a multi-layer epithelium and an enhanced expression of tight junctions markers zonula occludens 1, claudin 1 and occludin. These results support the relevance of the developed approach to pursue the exploration and development of human corneas stored in ASM as a physiological pharmacological model. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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