| Autor: |
Isabel Quesada-Ávila, Jorge Alfaro-Montoya, Rodolfo Umaña-Castro, André Braga, Marvin Ramírez-Alvarado, Tomer Ventura |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Aquaculture Reports, Vol 37, Iss , Pp 102257- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2352-5134 |
| DOI: |
10.1016/j.aqrep.2024.102257 |
| Popis: |
In this study, we further investigate the hypothesis that serotonin plus spiperone (Ser+Spi) injection induces ovarian maturation and releases ovarian maturation inducing pheromones into the water, thereby stimulating neighboring untreated females. Litopenaeus vannamei females (b.w. = 40.40 ± 5.84 g; n = 120) and males (b.w. = 34.25 ± 4.68 g; n= 90) were divided into three 18-m2 maturation tanks. Each tank contained 40 non-ablated females and 30 males with two treatments per tank. In tank 1, 20 females received a low dose of Ser+Spi (Ser at 25 µg g-1 b.w. and Spi at 2.0 µg g-1 b.w.) along with a control vehicle-injection. In tank 2, 20 females received a high dose of Ser+Spi (Ser at 40 µg g-1 b.w. and Spi at 4.0 µg g-1 b.w.) with the same control vehicle-injection applied to tank 1. In tank 3, unilateral eyestalk ablation (ESA) was performed on 20 females, while 20 control females were maintained non-ablated/injected. The experiment spanned 18 weeks and was divided into three phases. Ovarian maturation was observed in both Ser+Spi-treated and control females, with stages III-IV of maturation occurring one week after injections at both tested doses. Statistical analysis revealed that the average ovarian maturation index (OMI) was significantly higher for the control group in tank 2 (OMI = 0.25), compared to the Ser40+Spi4.0 group. Additionally, control females in tank 1 matured similarly to Ser25+Spi2.0 group (p ≤ 0.05). The control group of tank 3 displayed very low maturation activity (OMI = 0.1). Controls from tanks 1 and 2 exhibited a higher number of mated females and spawning events. These results support the potential of Ser+Spi to induce ovarian maturation and spawning and suggest that Ser+Spi-treated females may release a metabolite into the water that acts as an ovarian maturation inducer for neighboring females. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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