Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells
| Autor: | Jose A. Rodriguez, Rui Xu, Chien-Chun Chen, Zhifeng Huang, Huaidong Jiang, Allan L. Chen, Kevin S. Raines, Alan Pryor Jr, Daewoong Nam, Lutz Wiegart, Changyong Song, Anders Madsen, Yuriy Chushkin, Federico Zontone, Peter J. Bradley, Jianwei Miao |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | IUCrJ, Vol 2, Iss 5, Pp 575-583 (2015) |
| Druh dokumentu: | article |
| ISSN: | 2052-2525 20522525 |
| DOI: | 10.1107/S205225251501235X |
| Popis: | A structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 keV X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and the three-dimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. It is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres. |
| Databáze: | Directory of Open Access Journals |
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