Promoting Proliferation of Streptococcus thermophilus FUA329 and Antioxidant Activity of Enzyme Hydrolysis Products of Larimichthys polyactis

Autor: Xiaoyue LU, Ziyan HUA, Qing WU, Hao WU, Shu LIU, Guang YANG, Xiaoyue HOU, Yaowei FANG
Jazyk: čínština
Rok vydání: 2024
Předmět:
Zdroj: Shipin gongye ke-ji, Vol 45, Iss 18, Pp 138-145 (2024)
Druh dokumentu: article
ISSN: 1002-0306
46349928
DOI: 10.13386/j.issn1002-0306.2023110147
Popis: To determine the promoting effects of enzyme hydrolysis products of Larimichthys polyactis on the proliferation of Streptococcus thermophilus FUA329. The in vitro antioxidant activity of the enzymatic hydrolysis with different molecular weights were determined. Papain was selected for the enzymatic hydrolysis of its by-products on the basis of selecting different enzymes to hydrolyze the processing by-products of Larimichthys polyactis. Taking the proliferative effect of enzymatic degradation products on Streptococcus thermophilus as an indicator of evaluation. The enzymatic hydrolysis conditions were optimized through single factor and response surface methodology. The in vitro antioxidant activity of the hydrolysis products of the by-products of Larimichthys polyactis (HPBL) were determined. The results showed that the best enzymatic hydrolysis conditions included the hydrolysis time, material-liquid ratio and enzyme loading were 5 h, 1:5.1 (w/v) and 2.8% (w/v), respectively. The HPBL prepared under the optimized conditions was separated by ultrafiltration into four fragments, according to the molecular weight: >10000 Da, 5000~10000 Da, 3000~5000 Da,
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