| Autor: |
DANG Guang-lei, ZHANG Xiang-jin, ZHANG Jian-min, CHEN Hui, HE Wei |
| Jazyk: |
čínština |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Jichu yixue yu linchuang, Vol 40, Iss 5, Pp 609-614 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1001-6325 |
| Popis: |
Objective To investigate the effect of IFN-α1b on cytotoxic activity of human γδT cells against Daudi cells. Methods Daudi cells treated with IFN-α1b were functioned as target cells and γδT cells treated with IFN-α1b as effector cells. The cytotoxic activity of γδT cells was tested by a lactate dehydrogenase release assay. ELISA kits were applied to detect the level of granzymes B and IFN-γ released from γδT cells. Flow cytometry was performed for examining Fas receptor, stress protein ligands on Daudi cells and activation markers CD69 and CD25 of γδT cells. Results Both IFN-α1b pretreated Daudi cells or γδT cells significantly augmented the cytotoxic activity of γδT cells against Daudi cells (P＜0.0001). IFN-α1b also enhanced granzymes B and IFN-γ secretion of γδT cells(P＜0.05); IFN-α1b significantly up-regulated the expression of Fas receptor and stress ligand ULBP4 on but not ULBP3 on Daudi cells; IFN-α1b also increased the expression of CD69 but not CD25 on γδT cells. Conclusions IFN-α1b improves cytotoxicity of human γδT cells against cancer cell line Daudi by different mechanisms.So it is believed that IFN-α1b plus γδT cells may enhance the anti-tumor effect of γδT cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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