| Autor: |
Božić Nataša, Dojnov Biljana, Milovanović Aleksandra, Nenadović Vera, Ivanović Jelisaveta, Vujčić Z. |
| Jazyk: |
angličtina |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Archives of Biological Sciences, Vol 60, Iss 3, Pp 403-409 (2008) |
| Druh dokumentu: |
article |
| ISSN: |
0354-4664 |
| DOI: |
10.2298/ABS0803403B |
| Popis: |
Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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