| Autor: |
WU Tianyu, SONG Yalan, WU Jingfeng, ZHENG Zhiyuan |
| Jazyk: |
čínština |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
陆军军医大学学报, Vol 45, Iss 19, Pp 1995-2006 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2097-0927 |
| DOI: |
10.16016/j.2097-0927.202307121 |
| Popis: |
Objective To investigate the effects of interleukin22 (IL22) in acute colitis induced by dextran sulfate sodium salt (DSS) in mice and its underlying mechanism. Methods Three IL22 knockout mice (IL22-/-) and 3 control mice (IL22+/+) of 8-week-old males were subjected from the same litter, and then had free-choice drinking of 2.5% DSS water for 6 d followed by normal water for 2 d to construct a mouse model of acute colitis. The changes in body mass weight and disease activity index (DAI) were measured and calculated. After the intervention of 8 d, mouse enteroscopy was performed, and the length of colon and the organ index of major immune organs were measured in both groups. Protein levels of pro-inflammatory cytokines, such as IL1α, IL1β, IL6 and TNFα were detected by Western blotting. Adeno-associated virus (AAV) transfection model was established in 8-week-old male C57BL/6 mice, which were then treated by 2.5%DSS drinking. The mice were randomly divided into PBS group (n=5), AAV-mock+DSS group (n=5) and AAV-IL22+DSS group (n=5). The expression levels of colonic closed small loop protein 1 (ZO-1) and calcium adhesion protein (E-claudin) in different groups were detected by immunofluorescence assay. The protein levels of IL22, Occludin, Stat3 and p-Stat3 were detected by Western blotting. Results Compared with the IL22+/+ group, the mice in the IL22-/- group had significantly decreased body weight on day 8 (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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