Estimation of salivary proteins in early childhood caries before and after treatment using gel electrophoresis
| Autor: | Ravneet Kaur, H P Suma Sogi, Prinka Shahi, Sidhant Pathak, Mansi Jain, Tanvir Sidhu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Journal of Indian Society of Pedodontics and Preventive Dentistry, Vol 39, Iss 4, Pp 403-408 (2021) |
| Druh dokumentu: | article |
| ISSN: | 0970-4388 1998-3905 |
| DOI: | 10.4103/jisppd.jisppd_452_20 |
| Popis: | Background: Saliva being an important biological fluid of our body contains both specific and nonspecific protective factors which form an integral part of our immune system. Salivary proteins play a substantial role in protecting humans against infection. Their level in oral cavity is subject to constant variations which is dependent on various factors. Purpose: The purpose of the study was to compare the levels of salivary proline-rich proteins (PRPs), glycoproteins, amylase bands, and salivary pH in children with early childhood caries before and after treatment using gel electrophoresis. Materials and Methods: The whole salivary pH, mean protein concentrations, and electrophoretic profiles of the salivary proteins were measured using sodium dodecylsulfate–polyacrylamide gel electrophoresis in both pre- and posttreatment groups. Statistical analysis was done using Statistical Package for Social Sciences (SPSS) version 15.0 software. Chi-square test and independent t-test were used to further compare the results. Results: The results were statistically significant in all the groups. There was variation in pre- and posttreatment values seen. Conclusion: Salivary proteins (glycoproteins, PRPs, and amylase) establish an imperative defense mechanism as antimicrobial agents. |
| Databáze: | Directory of Open Access Journals |
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