Processing and reporting of cytology specimens from mediastinal lymph nodes collected using endobronchial ultrasound-guided transbronchial needle aspiration: a state-of-the-art review

Autor: Inderpaul Singh Sehgal, Nalini Gupta, Sahajal Dhooria, Ashutosh Nath Aggarwal, Karan Madan, Deepali Jain, Parikshaa Gupta, Neha Kawatra Madan, Arvind Rajwanshi, Ritesh Agarwal
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Journal of Cytology, Vol 37, Iss 2, Pp 72-81 (2020)
Druh dokumentu: article
ISSN: 0970-9371
DOI: 10.4103/JOC.JOC_100_19
Popis: Endobronchial ultrasound (EBUS)-guided transbronchial needle aspiration (TBNA) is presently the preferred modality for sampling mediastinal lymph nodes. There is an unmet need for standardization of processing and reporting of cytology specimens obtained by EBUS-TBNA. The manuscript is a state-of-the-art review on the technical aspects of processing and reporting of EBUS-TBNA specimens. A literature search was conducted using the PubMed database, and the available evidence was discussed among the authors. The evidence suggests that at least one air-dried and one alcohol-fixed slide should be prepared from each lymph node pass. The remaining material should be utilized for microbiological analysis (in saline) and cell block preparation (10% formalin or other solutions). Wherever available, rapid-onsite evaluation should be performed to assess the adequacy of the sample and guide the need for additional material. The lymph node aspirate should also be collected in Roswell Park Memorial Institute solution in cases where lymphoma is under consideration. The use of liquid-based cytology provides good quality specimens that are free from blood and air-drying artifacts and can be used wherever available. Sample adequacy and the diagnostic category should be furnished separately in the cytology report.
Databáze: Directory of Open Access Journals
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