| Autor: |
Aida Abdeen Mahmoud, Abdellah Hamed Khalil Ali, Essam Nour Eldin |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Egyptian Journal of Medical Human Genetics, Vol 17, Iss 2, Pp 191-196 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
1110-8630 |
| DOI: |
10.1016/j.ejmhg.2015.12.002 |
| Popis: |
Background: Sirtuin-1 (SIRT-1), a protein has been found to protect the cells against oxidative stress due to its deacetylase activity. In this investigation, we aimed to study SIRT-1 gene rs2273773 C > T single nucleotide polymorphism and markers of serum protein oxidation (protein carbonyl and sulfhydryl groups) in asthmatic patients. Subjects and methods: 120 asthmatic patients and 120 healthy controls were genotyped for SIRT-1 gene rs2273773 C > T SNP using polymerase chain reaction – confronting two pair primer method (PCR-CTPP). Serum protein carbonyl and sulfhydryl groups were measured using colorimetric methods. Results: SIRT-1 gene rs2273773 C > T SNP genotyping revealed that the TT genotype was significantly higher in the patients compared to the controls (P 0.05). T allele was significantly higher in the patients compared to the controls (P = 0.017). The distribution of the genotypes didn’t differ among the atopic and the non-atopic asthmatic patients, also no difference was found in the genotype distribution according to the severity of asthma (P > 0.05). Serum protein carbonyl group concentration was significantly higher in the patients compared to the controls (P T genotype were found. Conclusion: SIRT-1 gene rs2273773 C > T SNP was associated with asthma but not with protein oxidation markers in Egyptian population. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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