Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus

Autor: Shanthi Jayawardena, Chung Y. Cheung, Ian Barr, Kwok H. Chan, Honglin Chen, Yi Guan, J.S. Malik Peiris, Leo L.M. Poon
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Zdroj: Emerging Infectious Diseases, Vol 13, Iss 6, Pp 899-901 (2007)
Druh dokumentu: article
ISSN: 1080-6040
1080-6059
DOI: 10.3201/eid1306.061572
Popis: We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.
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