| Autor: |
Ana Helena Macedo Pereira, Alisson Campos Cardoso, Kleber Gomes Franchini |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
STAR Protocols, Vol 2, Iss 4, Pp 100950- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2021.100950 |
| Popis: |
Summary: Isolation and culture of ventricular cardiomyocytes from neonatal rats (NRVMs) is a powerful model to study neonatal cardiac development, cell cycle regulation, and cardiac physiology and pathology in vitro. Here, we present our modified enzymatic digestion protocol followed by two-step discontinuous Percoll gradient centrifugation to isolate a high yield of viable ventricular cardiomyocytes from neonatal rats. Finally, here we describe an immunostaining protocol for cytosolic and nuclear staining of NRVMs.For complete details on the use and execution of this protocol, please refer to Pereira et al. (2020). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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