A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function

Autor: Casey Crooks, Nathan J. Bechle, Franz J. St John
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Frontiers in Molecular Biosciences, Vol 8 (2021)
Druh dokumentu: article
ISSN: 2296-889X
DOI: 10.3389/fmolb.2021.714238
Popis: The Acetivibrioclariflavus (basonym: Clostridium clariflavum) glycoside hydrolase family 30 cellulosomal protein encoded by the Clocl_1795 gene was highly represented during growth on cellulosic substrates. In this report, the recombinantly expressed protein has been characterized and shown to be a non-reducing terminal (NRT)-specific xylobiohydrolase (AcXbh30A). Biochemical function, optimal biophysical parameters, and phylogeny were investigated. The findings indicate that AcXbh30A strictly cleaves xylobiose from the NRT up until an α-1,2-linked glucuronic acid (GA)-decorated xylose if the number of xyloses is even or otherwise a single xylose will remain resulting in a penultimate GA-substituted xylose. Unlike recently reported xylobiohydrolases, AcXbh30A has no other detectable hydrolysis products under our optimized reaction conditions. Sequence analysis indicates that AcXbh30A represents a new GH30 subfamily. This new xylobiohydrolase may be useful for commercial production of industrial quantities of xylobiose.
Databáze: Directory of Open Access Journals