Respiratory metabolites in bronchoalveolar lavage fluid (BALF) and exhaled breath condensate (EBC) can differentiate horses affected by severe equine asthma from healthy horses

Autor: Marilena Bazzano, Luca Laghi, Chenglin Zhu, Gian Enrico Magi, Beniamino Tesei, Fulvio Laus
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: BMC Veterinary Research, Vol 16, Iss 1, Pp 1-9 (2020)
Druh dokumentu: article
ISSN: 1746-6148
DOI: 10.1186/s12917-020-02446-9
Popis: Abstract Background The use of an untargeted metabolomic approach to investigate biofluids of respiratory origin is of increasing interest in human and veterinary lung research. Considering the high incidence of equine asthma (> 14%) within horse population and the importance of this animal model for human disease, we aimed to investigate the metabolomic profile of bronchoalveolar lavage fluid (BALF) and exhaled breath condensate (EBC) in healthy and asthmatic horses. Results On the basis of clinical, endoscopic and BALF cytology findings, 6 horses with severe asthma (Group A) and 6 healthy horses (Group C) were included in the study. 1H-NMR analysis was used to identified metabolites in BALF and EBC samples. Metabolomic analysis allowed to identify and quantify 12 metabolites in BALF and seven metabolites in EBC. Among respiratory metabolites, myo-inositol, formate, glycerol and isopropanol in BALF, and methanol and ethanol in EBC, differed between groups (p
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