System-wide identification and prioritization of enzyme substrates by thermal analysis
| Autor: | Amir Ata Saei, Christian M. Beusch, Pierre Sabatier, Juan Astorga Wells, Hassan Gharibi, Zhaowei Meng, Alexey Chernobrovkin, Sergey Rodin, Katja Näreoja, Ann-Gerd Thorsell, Tobias Karlberg, Qing Cheng, Susanna L. Lundström, Massimiliano Gaetani, Ákos Végvári, Elias S. J. Arnér, Herwig Schüler, Roman A. Zubarev |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2041-1723 24403687 |
| DOI: | 10.1038/s41467-021-21540-6 |
| Popis: | The global identification of enzyme substrates is still challenging. Here, the authors develop a method based on proteome-wide thermal shift assays to discover enzyme substrates directly from cell lysates, identifying known and novel oxidoreductase, kinase and poly-(ADP-ribose) polymerase substrates. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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