Preventive Supplementation of Omega-3 Reduces Pain and Pro-inflammatory Cytokines in a Mouse Model of Complex Regional Pain Syndrome Type I

Autor: Taynah de Oliveira Galassi, Paula Franson Fernandes, Afonso Shiguemi Inoue Salgado, Francisco José Cidral-Filho, Anna Paula Piovezan, Daniela Dero Lüdtke, Josiel Mileno Mack, Kenneth A. Weber, William R. Reed, Franciane Bobinski, Daniel F. Martins
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Frontiers in Integrative Neuroscience, Vol 16 (2022)
Druh dokumentu: article
ISSN: 1662-5145
77442466
DOI: 10.3389/fnint.2022.840249
Popis: Complex regional pain syndrome type I (CRPS-I) is a condition that responds poorly to treatments. The role of omega-3 fatty acids in the treatment of inflammatory disorders is well described in the literature; however, few studies have evaluated its therapeutic benefits in different types of pain. We evaluated the potential antihyperalgesic and anti-inflammatory effects of preventive omega-3 supplementation in an animal model of CRPS-I. In experiment 1, Swiss female mice were supplemented for 30 days with omega-3 before the induction of the CRPS-I model and 14 days after. Mechanical hyperalgesia was evaluated at baseline and from the 4th to the 14th day after CPRS-I induction along with open field locomotor activity after 30 days of supplementation. In experiment 2, Swiss female mice were supplemented for 30 days with omega-3 and then subjected to the CRPS-I model. Twenty-four hours later the animals were euthanized, and tissue samples of the spinal cord and right posterior paw muscle were taken to measure pro-inflammatory cytokine TNF and IL-1β concentrations. Omega-3 supplementation produced antihyperalgesic and anti-inflammatory effects, as well as reducing pro-inflammatory cytokine concentrations, without altering the animals’ locomotion. No open field locomotor changes were found. The 30-day supplementation at the tested dose was effective in the CRPS-I model.
Databáze: Directory of Open Access Journals
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