Validation of Half-Reaction Volumes of the Promega PowerPlex® Forensic Amplification Kits (PowerPlex® 18D Systems, PowerPlex ® 21System, PowerPlex® Fusion System and PowerPlex® Y23 System) in STR Analysis
| Autor: | Hanan K. Mahmood, Nadia F. Salman, Dhurgham H. Hasan, Khaleefah M. Salih, Maryam A. Sadiq, Basma T. Mohammad, Maryam K. Mohammed, Sarwa M. Nahi, Sara S. Baqir |
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| Jazyk: | Arabic<br />English |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Arab Journal of Forensic Sciences & Forensic Medicine, Vol 2, Iss 2, Pp 152-158 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1658-6786 1658-6794 |
| DOI: | 10.26735/QFSI2426 |
| Popis: | DNA amplification is known to be the most expensive step during forensic DNA analysis. This study evaluated the half-reaction amplification protocol (12.5 µL PCR product) using DNA amplification kits from Promega PowerPlex® (PowerPlex® 18D System, PowerPlex ®21System, PowerPlex® Fusion System and PowerPlex® Y23 System), which might aid in reducing sample analysis cost by half and allow the analysis of more samples. A sensitivity study (15 samples) along with testing of various blood stain samples (n=100) that were submitted to the Medico-Legal Directorate laboratory for DNA testing was accomplished to compare the DNA profiles resulting from half-reaction volume procedure to those with full-reaction volume procedure, using three differed methods along with standard protocol to evaluate the effect of half reaction volume with some variables. Results demonstrated the use of half-reaction amplification protocol preceded by washing step for all aforementioned DNA amplification kits gave a robust and reliable amplification result that aid to increase the number of samples analyzed and decreased the test cost for each kit without compromising the quality of 3DNA profiles obtained. |
| Databáze: | Directory of Open Access Journals |
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