FSH and LH Secretion from in-vitro Cultured Buffalo Anterior Pituitary Cells Following Treatment with Diethyl-Stilbestrol and Medroxy-Progesterone and Their Effects on Ovarian Activity and Hematological Variables of Female Rabbits
| Autor: | Kaleem Iqbal1, Nafees Akhtar1*, Nazir Ahmad1 and Sajjad-ur-Rahman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Pakistan Veterinary Journal, Vol 36, Iss 4, Pp 445-449 (2016) |
| Druh dokumentu: | article |
| ISSN: | 0253-8318 2074-7764 |
| Popis: | Aims of this study were: to investigate whether FSH and LH secretion from in-vitro cultured buffalo adenohypophyseal cells can be increased by supplementing culture media with diethyl-stilbestrol and medroxy-progesterone, respectively; to monitor bioactivity of these in-vitro produced gonadotropins and to see if these gonadotropins have any adverse effects on hematology and internal body organs of female rabbits. Pituitary glands collected from 36 adult buffaloes slaughtered at a local abattoir were used. The anterior pituitary cells were cultured in-vitro using medium RPMI-1640 (code R6504-Sigma) enriched with 10% fetal calf serum and GnRH and treated with 0.5 or 1.0 mg/100 ml diethyl-stilbestrol, and 2.5 or 5.0 mg/ml medroxy-progesterone, or left as untreated control. The results showed that FSH and LH concentrations from cultures treated with low or high dose of respective steroids were higher (P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|