In vitro production of porcine embryos with use of chemically semi-defined culture media system
| Autor: | David Urbán Duarte, Horacio Álvarez Gallardo, Sandra Pérez Reynozo, José Fernando De la Torre Sánchez |
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| Jazyk: | English<br />Spanish; Castilian |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Revista Mexicana de Ciencias Pecuarias, Vol 11, Iss 1, Pp 102-115 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2007-1124 2448-6698 |
| DOI: | 10.22319/rmcp.v11i1.4757 |
| Popis: | The objective of this study was to determine the effect of a semi-defined culture media system developed in laboratory, named Pigs Media System (PMS) on the in vitro production of porcine embryos. In a first assay, the cummulus-oocytes complexes (COCs) were matured, fertilized and cultured for embryo development in PMS supplemented with bovine serum albumin (BSA), and in North Carolina State University-23 (NCSU-23) supplemented with follicular fluid, until blastocysts evaluation. In the assay 2, maturation and culture were performed in PMS using BSA or polyvinyl alcohol (PVA) in a 2 × 2 factorial arrangement (PMS-BSA/BSA, PMS-BSA/PVA, PMS-PVA/PVA, PMS-PVA/BSA). The PMS had a positive effect on the total cell number (58.04) and the decrease of the total lipids (49.4 %) regarding the NCSU-23 medium (37.98 and 59.2 % respectively; P |
| Databáze: | Directory of Open Access Journals |
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