Experimental estimation of the effects of exogenous carbon monoxide on blood cells

Autor: I. V. Petrova, J. G. Birulina, O. A. Trubacheva, S. N. Belyaeva, O. L. Shnaider, A. V. Nosarev, S. V. Gusakova, V. N. Vasilev, G. A. Suhanova
Jazyk: English<br />Russian
Rok vydání: 2020
Předmět:
Zdroj: Бюллетень сибирской медицины, Vol 19, Iss 1, Pp 94-100 (2020)
Druh dokumentu: article
ISSN: 1682-0363
1819-3684
DOI: 10.20538/1682-0363-2020-1-94-100
Popis: The aim of the study was to investigate the effect of the carbon monoxide (CO) donor on the Ca2+-activated potassium permeability of the erythrocyte membrane and platelet aggregation ability.Materials and methods. Healthy volunteers (n = 27) and patients with chronic coronary heart disease (CHD) (n = 32) of both sexes were examined. The material of the study was packed red blood cells and platelet-rich plasma obtained from patient’s venous blood. The change of Ca2+-dependent potassium conductivity of the erythrocyte membrane was evaluated by potentiometric method, and the platelet aggregation was studied by turbidimetric method. Carbon monoxide releasing molecule-2 (CORM-2) was used as a CO donor. The amplitude of A23187- and redox-induced hyperpolarization response (HR) of erythrocytes, and the rate and degree of platelet aggregation were estimated.Results. It was shown that the addition of CORM-2 (10 and 100 μM) in the erythrocyte suspension caused a dose-dependent decrease in the amplitude of A23187- and redox-dependent HR in healthy donors, as well as in patients with chronic CHD. The maximum decrease was observed in the presence of 100 μM CORM-2. The effect of CORM-2 at concentrations of 10 and 100 μM on collagen-induced platelet aggregation led to a decrease in the degree and rate of aggregation in healthy donors. The maximum effect was shown at 100 μM of CO donor. However, such an unambiguous effect of CORM-2 on the aggregation parameters in patients with CHD was not observed.Conclusion. The results suggest that CO has a significant effect on the ion transport function of the erythrocyte membrane and platelet aggregation activity of both healthy donors and patients with CHD.
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