| Autor: |
Chi Zhang, Qian Huang, Neil C Ford, Nathachit Limjunyawong, Qing Lin, Fei Yang, Xiang Cui, Ankit Uniyal, Jing Liu, Megha Mahabole, Hua He, Xuewei Wang, Irina Duff, Yiru Wang, Jieru Wan, Guangwu Zhu, Srinivasa N Raja, Hongpeng Jia, Dazhi Yang, Xinzhong Dong, Xu Cao, Scheffer C Tseng, Shaoqiu He, Yun Guan |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
eLife, Vol 13 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2050-084X |
| DOI: |
10.7554/eLife.101269 |
| Popis: |
Pain after surgery causes significant suffering. Opioid analgesics cause severe side effects and accidental death. Therefore, there is an urgent need to develop non-opioid therapies for managing post-surgical pain. Local application of Clarix Flo (FLO), a human amniotic membrane (AM) product, attenuated established post-surgical pain hypersensitivity without exhibiting known side effects of opioid use in mice. This effect was achieved through direct inhibition of nociceptive dorsal root ganglion (DRG) neurons via CD44-dependent pathways. We further purified the major matrix component, the heavy chain-hyaluronic acid/pentraxin 3 (HC-HA/PTX3) from human AM that has greater purity and water solubility than FLO. HC-HA/PTX3 replicated FLO-induced neuronal and pain inhibition. Mechanistically, HC-HA/PTX3-induced cytoskeleton rearrangements to inhibit sodium current and high-voltage activated calcium current on nociceptive DRG neurons, suggesting it is a key bioactive component mediating pain relief. Collectively, our findings highlight the potential of naturally derived biologics from human birth tissues as an effective non-opioid treatment for post-surgical pain. Moreover, we unravel the underlying neuronal mechanisms of pain inhibition induced by FLO and HC-HA/PTX3. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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