Evaluation of the Ankylosing Spondylitis Transcriptome for Oxidative Phosphorylation Pathway: The Shared Pathway with Neurodegenerative Diseases
| Autor: | Arezou Lari, Hamid Gholami Pourbadie, Ali Sharifi-Zarchi, Saeed Aslani, Leila Nejatbakhsh Samimi, Ahmadreza Jamshidi, Mahdi Mahmoudi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Iranian Journal of Allergy, Asthma and Immunology, Vol 20, Iss 5 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1735-1502 1735-5249 |
| DOI: | 10.18502/ijaai.v20i5.7406 |
| Popis: | Ankylosing spondylitis (AS) is a systemic inflammatory disorder of joints and entheses. Recent studies have reported an increased prevalence of dementia in AS patients. However, data for exploring the association between dementia and AS remain uncertain. In this study, enriched pathways and differentially expressed genes (DEGs) were identified in whole blood transcription data of AS patients obtained from the gene expression omnibus (GEO) database; using gene set enrichment analysis (GSEA) and differential expression analysis. Four pathways, including oxidative phosphorylation, Alzheimer’s, Parkinson’s, and Huntington’s diseases were significantly enriched in AS patients compared to the controls. We identified 22 common genes among the pathways that showed an increasing trend in AS compared to the controls. Five of them including COX7B, NDUFB3, ATP5PF, UQCRB, and NDUFS4 were the most significant genes which were selected for gene expression analysis; using real-time PCR on RNA contents of peripheral blood mononuclear cells (PBMCs) of AS patients and controls (20 samples from each group). The gene expression analysis indicated considerable overexpression of COX7B (p |
| Databáze: | Directory of Open Access Journals |
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