Association of human interleukin-35 level in gingival crevicular fluid and serum in periodontal health, disease, and after nonsurgical therapy: A comparative study
| Autor: | Subash Chandra Raj, Subhashree Manaswini Panda, Muktikesh Dash, Kaushik Patnaik, Devapratim Mohanty, Neelima Katti, Annuroopa Mahapatra, Debasish Mishra, Kamdev Praharaj |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Contemporary Clinical Dentistry, Vol 9, Iss 2, Pp 293-297 (2018) |
| Druh dokumentu: | article |
| ISSN: | 0976-237X 0976-2361 |
| DOI: | 10.4103/ccd.ccd_51_18 |
| Popis: | Background: Innovating newer methods to diagnose a multifactorial disease such as periodontitis is always challenging for a clinician. Gingival crevicular fluid (GCF) which is closely associated with the periodontal tissue environment has been used a viable alternative to saliva for the diagnosis of periodontitis. Aim: The aim of the present study was to estimate and compare the interleukin-35 (IL-35) levels in GCF and serum among healthy, gingivitis, and chronic periodontitis (CP) individuals as well as to evaluate the effect of nonsurgical periodontal treatment (NSPT) on IL-35 level among patients with CP. Settings and Design: The study was conducted at the Department of Periodontics, Srirama Chandra Bhanja Dental College and Hospital, Cuttack, Odisha, India. It is a comparative study. Materials and Methods: A total of 60 participants were divided into healthy (Group I; n = 20), gingivitis (Group II; n = 20), and CP (Group IIIA; n = 20). GCF samples collected from each individual at baseline and 6 weeks after NSPT for Group III individuals (Group IIIB; n = 20) were quantified for IL-35 levels using enzyme-linked immunosorbent assay. Statistical Analysis: All analyses were performed using Shapiro–Wilk test, analysis of variance, Tukey's honestly significant difference post hoc test, and multiple regression analysis. Results: The mean IL-35 concentration in GCF was significantly high (P < 0.05) for Group IIIA (70.26 ± 4.0 pg/ml), as compared to Group I (54.81 ± 22.3 pg/ml) and Group IIIB (55.72 ± 10.2 pg/ml). Conclusion: In the present study, GCF and serum IL-35 concentration among CP individuals was highest among all the groups. Individuals receiving NSPT showed a significant reduction in IL-35 levels as compared to CP individuals. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|