| Autor: |
Mohit Kumar, Apoorv Pant, Rohit Bansal, Ashutosh Pandey, James Gomes, Kedar Khare, Anurag Singh Rathore, Manidipa Banerjee |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Computational and Structural Biotechnology Journal, Vol 18, Iss , Pp 1458-1465 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2001-0370 |
| DOI: |
10.1016/j.csbj.2020.06.009 |
| Popis: |
Aggregation is a critical parameter for protein-based therapeutics, due to its impact on the immunogenicity of the product. The traditional approach towards characterization of such products is to use a collection of orthogonal tools. However, the fact that none of these tools is able to completely classify the distribution and physical characteristics of aggregates, implies that there exists a need for additional analytical methods. We report one such method for characterization of heterogeneous population of proteins using transmission electron microscopy. The method involves semi-automated, size-based clustering of different protein species from micrographs. This method can be utilized for quantitative characterization of heterogeneous populations of antibody/protein aggregates from TEM images of proteins, and may also be applicable towards other instances of protein aggregation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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