Toxicity of Pre-heated Composites Polymerized Directly and Through CAD/CAM Overlay

Autor: Alena Knezevic, Davor Zeljezic, Nevenka Kopjar, Sillas Duarte, Jr., Matej Par, Zrinka Tarle
Jazyk: English<br />Croatian
Rok vydání: 2018
Předmět:
Zdroj: Acta Stomatologica Croatica, Vol 52, Iss 3, Pp 203-217 (2018)
Druh dokumentu: article
ISSN: 0001-7019
1846-0410
DOI: 10.15644/asc52/3/4
Popis: Objectives: The aim was to compare cytotoxicity/genotoxicity of pre-heated composites polymerized through CAD/CAM overlays on isolated human peripheral blood lymphocytes. Material and Methods: A microhybrid (Z100, 3M ESPE) and nanofilled composite (Filtek Supreme Ultra, 3M ESPE) were heated in a heating unit (Calset, AdDent Inc.) at different temperatures: 37 oC, 54 oC, and 68 oC. A small amount of heated composite was placed in a cylindrical mold (6mm diameter; 0.65mm thick), covered with a Mylar sheet, pressed and light-cured directly and through 2 mm thick CAD/CAM ceramic-reinforced polymer (CRP)(LAVA Ultimate, 3M ESPE) or CAD/CAM lithium disilicate ceramic (LDC)(e.max, Ivoclar/Vivadent) overlay. After curing, the specimens were immediately placed in a prepared lymphocyte cell culture. Cytotoxicity was assessed using a dye exclusion method by simultaneous staining with ethidium bromide and acridine orange, aimed to determine percentages of viable, apoptotic and necrotic cells. Genotoxicity was studied using alkaline comet assay. Results: For Z100, the highest percentage of viable cells is recorded at T1 (93.7%) after direct light curing, followed by light curing through CRP (92.3%) and through LDC (91.7%T1,T3). For Filtek Supreme Ultra, the highest percentage of viable cells is recorded while curing through CRP (91.0% T2), followed by LDC (90% T1,T3) and direct light curing (88.7%T2). Conclusion: For both tested materials, preheating the procedure at T1 and T2 may be the procedure of choice. In terms of genotoxicity, reheating at T3 may not be suggested.
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