| Autor: |
Christiane D'Oliveira Marques, Luis Antonio dos Santos Diego, Jussara Marcondes-Machado, Renée Lauffer Amorim, Lídia Raquel Carvalho, Norma Sueli Pinheiro Módolo, Leandro Gobbo Braz, Yara Marcondes Machado Castiglia |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Acta Cirúrgica Brasileira, Vol 31, Iss 7, Pp 434-441 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
0102-8650 |
| DOI: |
10.1590/S0102-865020160070000002 |
| Popis: |
ABSTRACT PURPOSE: To investigate changes in the serum concentration and renal expression of IL-1 and TNF-α cytokines in rats that received sevoflurane and glibenclamide prior to hemorrhage. METHODS: Two groups of sevoflurane-anesthetized Wistar rats (n=10): G1 (control) and G2 (glibenclamide, 1 µg/g i.v.); hemorrhage of 30% blood volume (10% every 10 min), with replacement using Ringer solution, 5 ml/kg/h. Serum concentrations of IL-1 and TNF-α were studied in the first hemorrhage (T1) and 50 min later (T2), renal expression, at T2. RESULTS: In serum, G1 TNF-α (pg/mL) was T1=178.6±33.5, T2=509.2±118.8 (p0.05). The expression of TNF-α and IL-1 in the glomerular and tubular cells was significantly higher in the G2 group. CONCLUSIONS: Hemorrhage and glibenclamide elevated TNF-α and IL-1 concentrations in serum and kidneys. High levels of TNF-α already present before the hemorrhage in the glibenclamide group may have attenuated the damages found in the kidneys after the ischemia event. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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