| Autor: |
Kang Yangjoo, Kim Deuk-Su, Kim Kibum, Myung Soon-Chul, Oh Yoo Jin, Park Sungsu, Hinterdorfer Peter, Ko Kisung |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
The EuroBiotech Journal, Vol 7, Iss 1, Pp 87-95 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2564-615X |
| DOI: |
10.2478/ebtj-2023-0006 |
| Popis: |
Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain proteins were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic plants were crossed together to generate F1 expressing both PAP-IgM FcK and J-chain proteins (PAP-IgM FcK × J-chain). PCR and RT-PCR analyses confirmed the transgene insertion and mRNA transcription of PAP-IgM FcK and J-chain in leaf tissue of PAP-IgM FcK × J-chain F1 plant. Western blot confirmed the expression of PAP-IgM FcK × J-chain protein. Size exclusion (SEC)-high performance liquid chromatography (HPLC) and Bio-transmission electron microscope (TEM) analyses were performed to show the size and shape of the PAP- IgM FcK × J-chain fusion proteins. These results suggest that PAP-IgM FcK with J-chain can be produced in plant expression system with plant crossing. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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