A Microbiological Assay for the Quantitative Determination of Glutathione

Autor: Martin Schmidt, Martin Grey, Martin Brendel
Jazyk: angličtina
Rok vydání: 1996
Předmět:
Zdroj: BioTechniques, Vol 21, Iss 5, Pp 881-886 (1996)
Druh dokumentu: article
ISSN: 1940-9818
0736-6205
DOI: 10.2144/96215st07
Popis: Based on the requirement of a glutathione-deficient mutant strain of Saccharomyces cerevisiae to take up external glutathione for growth on synthetic media, a simple agar diffusion test for quantitative detection of total glutathione from various sources was established. Glutathione concentrations can be reliably detected in a less expensive way, requiring less technical effort compared to enzymatic or biochemical detection methods.
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