| Popis: |
I n this study, previously synthesized Ni II phthalocyanine compound bearing 2,10,16,24-tetrakis 2-isopropyl-5-methylphenoxy group was chosen for its interaction with calf thymus-DNA. Calf thymus-DNA was used to determine DNA binding properties of Ni II phthalocyanine compound. The DNA binding activities of Ni II phthalocyanine compound bearing 2-isopropyl-5-methylphenoxy substituent was investigated by using absorption titration, fluorescence emission, cyclic voltammetry, gel elctrophoresis in Tris-HCl buffer at pH 7.0. In addition to above methods, melting point and viscosity experiment were performed to determine the DNA intercation of the compound in Tris-HCl buffer solution. The results showed that Ni II phthalocyanine compound binds strongly to calf thymus-DNA via intercalation binding |
