Guidelines for quantifying leaf chlorophyll content via non‐destructive spectrometry

Autor: Giancarlo M. Chiarenza, Eve Slavich, Angela T. Moles
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Applications in Plant Sciences, Vol 12, Iss 6, Pp n/a-n/a (2024)
Druh dokumentu: article
ISSN: 2168-0450
DOI: 10.1002/aps3.11610
Popis: Abstract Premise Leaf chlorophyll is a fundamental bioindicator used in several fields; however, we lack clear guidelines for optimizing sampling efforts and producing comparable studies. Methods We estimated the leaf chlorophyll content of 10 plant species using nondestructive spectrometry methods. We stored half of the leaves at 4°C and half at room temperature under similar light levels to assess the role of storage in the chlorophyll degradation rate. Results The chilled mature leaves maintained a chlorophyll content within 5% of the original value for ~1.5 d, while the chlorophyll content of unrefrigerated mature leaves decreased rapidly, indicating that their chlorophyll content should be measured within 4 h. When refrigerated, the chlorophyll content of the expanding leaves remained within 5% of the original level for at least 5 d, but we suggest analyzing them within 3 d. In mature leaves, 73% of the variation in chlorophyll content is at the species level, 15% is between individuals, and the variation within leaves is negligible (
Databáze: Directory of Open Access Journals
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