Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes

Autor: Omar A Saldarriaga, Jaime I Velásquez, Jorge E Ossa, Maria T Rugeles
Jazyk: angličtina
Rok vydání: 2003
Předmět:
Zdroj: Memorias do Instituto Oswaldo Cruz, Vol 98, Iss 2, Pp 269-271 (2003)
Druh dokumentu: article
ISSN: 0074-0276
1678-8060
DOI: 10.1590/S0074-02762003000200017
Popis: We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.
Databáze: Directory of Open Access Journals
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