| Autor: |
Owen J. Harrison, Bertrand Monnier, Ed Regan, Dave West, Hugh Ballantine Dykes, Jeffrey S. Davey, Janice Kiely, Richard Luxton |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Sensing and Bio-Sensing Research, Vol 41, Iss , Pp 100566- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2214-1804 |
| DOI: |
10.1016/j.sbsr.2023.100566 |
| Popis: |
Expensive, time-consuming and labour-intensive solvent-extraction and liquid-chromatography methods are the industry's current gold standard for antibiotic residue quantification. A novel immunoassay methodology and system for the rapid detection of clinically relevant levels of tetracycline residues found in food-producing animal tissues is described. Anti-tetracycline antibody-coated paramagnetic particles were used for the specific capture of tetracycline in spiked buffer (with and without a 1% pork muscle tissue suspension) and quantified via an analytically-sensitive in-house magnetometer instrument. Detection of tetracycline between 0.1 μg/mL - 1 μg/mL was achieved, with a readout time (including sample treatment) presented in 20 min. The magneto-immunoassay described provides a rapid, low-cost, de-skilled and analytically-sensitive solution for tetracycline screening at the point-of-sampling, with potential applications for other prevalent antibiotic families used in the international farming and food industry. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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