Extraction and Titration of Leukotoxins from Fusobacterium necrophorum Isolates Recovered from Bovine Liver Abscesses in Sulaimaniyah Region

Autor: Suha Ali Hussein
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Zdroj: The Iraqi Journal of Veterinary Medicine, Vol 36, Iss 0E (2012)
Druh dokumentu: article
ISSN: 1609-5693
2410-7409
DOI: 10.30539/iraqijvm.v36i0E.403
Popis: This study was conducted to extract and titrate the leukotoxin of Fusobacterium necrophorum isolates recovered from 75abscesses found in 24 livers of slaughtered cattle in Sulaimaniyah region. The culture supernatants of these isolates were subjected to the tetrazolium dye reduction test which revealed that the leukotoxin titer values of 34 F. necrophorum subsp. necrophorum isolates ranged from 128 to 1024 (with a leukotoxin titer mean of 516±46), whereas the leukotoxin titer values of the 11 F. necrophorum subsp. funduliforme isolates ranged from 0 to 128 (with a leukotoxin titer mean of 73±12).
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