Generation of mixed murine organoids to model cellular interactions

Autor: Ana Krotenberg Garcia, Jacco van Rheenen, Saskia Jacoba Elisabeth Suijkerbuijk
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: STAR Protocols, Vol 2, Iss 4, Pp 100997- (2021)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100997
Popis: Summary: Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We designed a protocol to generate mixed murine organoids and enteroid monolayers used to study such complex cellular interactions in a mammalian system. This protocol is dedicated to follow the behavior of different cell populations over time, using (time-lapse) microscopy or transcriptome/proteome analysis.For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al. (2021).
Databáze: Directory of Open Access Journals