Effects of Nitrate Addition on Rumen Fermentation, Bacterial Biodiversity and Abundance

Autor:	Liping Zhao, Qingxiang Meng, Liping Ren, Wei Liu, Xinzhuang Zhang, Yunlong Huo, Zhenming Zhou
Jazyk:	angličtina
Rok vydání:	2015
Předmět:	Nitrate Rumen Fermentation Bacterial Community Ion Torrent Sequencing Quantitative Polymerase Chain Reaction Animal culture SF1-1100 Animal biochemistry QP501-801
Zdroj:	Asian-Australasian Journal of Animal Sciences, Vol 28, Iss 10, Pp 1433-1441 (2015)
Druh dokumentu:	article
ISSN:	1011-2367 1976-5517
DOI:	10.5713/ajas.15.0091
Popis:	This study examined changes of rumen fermentation, ruminal bacteria biodiversity and abundance caused by nitrate addition with Ion Torrent sequencing and real-time polymerase chain reaction. Three rumen-fistulated steers were fed diets supplemented with 0%, 1%, and 2% nitrate (dry matter %) in succession. Nitrate supplementation linearly increased total volatile fatty acids and acetate concentration obviously (p = 0.02; p = 0.02; p0.05) and beta biodiversity of ruminal bacteria were not affected by nitrate. Nitrate increased typical efficient cellulolytic bacteria species (Ruminococcus flavefaciens, Ruminococcus ablus, and Fibrobacter succinogenes) (p
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/5469a3b29c754ddd93a142845ec0b19e Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF