The analysis of DMD gene deletions by multiplex PCR in Indonesian DMD/BMD patients: the era of personalized medicine

Autor: Kristy Iskandar, Ery Kus Dwianingsih, Linda Pratiwi, Alvin Santoso Kalim, Hasna Mardhiah, Alifiani H. Putranti, Dian K. Nurputra, Agung Triono, Elisabeth S. Herini, Rusdy G. Malueka, Gunadi, Poh San Lai, Sunartini
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: BMC Research Notes, Vol 12, Iss 1, Pp 1-6 (2019)
Druh dokumentu: article
ISSN: 1756-0500
DOI: 10.1186/s13104-019-4730-1
Popis: Abstract Objective Duchenne/Becker muscular dystrophy (DMD/BMD) is the most common genetic neuromuscular disease in children, resulting from a defect in the DMD gene located on Xp21.2. The new emerging treatment using exon skipping strategy is tailored to specific mutations, thus molecular diagnostics are particularly important. This study aimed to detect the DMD gene deletion in Indonesian DMD/BMD patients and analyze the potential amenability by exon skipping therapy. Results Thirty-four male patients were enrolled in this study, 23 of them (67.6%) underwent muscle biopsy and showed the absence or partially expressed dystrophin protein in immunohistochemistry staining. All patients had very high serum CK levels (10.529 ± 9.97 IU/L). Multiplex PCR revealed the DMD gene deletions in 15 (44.1%) cases. Seventy-eight percent of deletions were clustered in the hot-spot region of exon 43 to 52. Furthermore, seven (20.5%) patients were potentially amenable to exon skipping treatment. Therefore, multiplex PCR is one feasible method to detect DMD gene deletion in Indonesian DMD/BMD patients that can further determine the potential amenability of exon skipping therapy. In addition, this study is the first report of DMD gene deletion analysis in Indonesia.
Databáze: Directory of Open Access Journals
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