Intestinal coccidiosis in children admitted to a hospital in Peru and comparison of two methods for detecting Cryptosporidium spp.
| Autor: | Heber Silva-Díaz, Hamer Campos-Flores, Jean Pier Llagas-Linares, Dunalia LLatas-Cancino |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Revista Peruana de Medicina Experimental y Salud Pública, Vol 33, Iss 4, Pp 739-744 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1726-4634 1726-4642 |
| DOI: | 10.17843/rpmesp.2016.334.2560 |
| Popis: | A cross-sectional study was done between October 2014 and June 2015 to estimate the frequency of infection due to intestinal coccidiosis in children admitted to a hospital in Peru, and compare the modified acid-fast staining procedure with the enzyme-linked immunoassay (ELISA) method for the detection of Cryptosporidium spp. Coccidia were detected using the modified acid-fast staining procedure and the Cryptosporidium ELISA method (kit r-Biopharm) on seriated stool samples. Out of a total of 325 children, 5.5% had some type of intestinal coccidiosis: 3.7% involved Cryptosporidium spp. (using both techniques) and 1.8% involved Cyclospora cayetanensis (using the modified acid-fast staining procedure). The modified acid-fast staining procedure and ELISA method revealed a 0.955 consistency in the detection of Cryptosporidium spp. In conclusion, intestinal coccidiosis is frequent in children based on the population studied; similarly, both techniques can be used to detect Cryptosporidium spp., but the modified acid-fast staining procedure has an advantage over the other because it is cost-effective and can detect other coccidia in everyday practice. |
| Databáze: | Directory of Open Access Journals |
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